Clipping is a handy way to collect important slides you want to go back to later. You can download the paper by clicking the button above. MATERIALS: If that all seems like a bit much, you arent wrong. To achieve more consistent tissue disruption across samples, you can also use a blender, though this step is still low throughput and time consuming. (PDF) CTAB -Extraction method in Plant tissue - ResearchGate Access Free The Cell A Molecular Approach Geoffrey M Cooper - ahecdata.utah A method for DNA Extraction and molecular identification of Aphids. %PDF-1.4 % 1. Increasing the heat and duration of the CTAB step might help with tough cells (eg. May need to be heated to 50C to dissolve. Basic steps for DNA extraction 3. It is a cationic detergent, and water and alcohol soluble. It involves breaking open the cells, removing proteins and other contaminants, and purifying the DNA so that it is free of other cellular components. Population genomic analyses of the chocolate tree, Theobroma cacao L., provide insights into its domestication process. PDF NA extraction: omparison of methodologies - NBPGR 0000000016 00000 n (1991) used 0.5% SDS with the CTAB buffer and developed a simple plant DNA extraction protocol. Inorganic DNA Extraction: 6M . Plant genomic DNA extraction - YouTube iKjA3)])"ZcaR*.&p)n5x.9,Vjt_* K;lZe0# ;Iv1WUbg>s{x,4L4(@[xidmy)dK4^Cv$[1` 8[O16Gok9$pZBQM'!(H0RK9ra)"}Vjo7`/J1j#3g3]( eo; M(]+d[` mAQ8 rk-%wURl0]vcRBqR^W(1+.Ho)T-^gbrGvVp-{x%`1wE;b,uD^Y{_o Our binding chemistry, wash solutions, and spin column technology remove polysaccharides, lipids, and other common downstream inhibitors and contaminants providing ultra-pure DNA, with minimal loss. Zymos Quick-DNA Plant/Seed kits use bead beating and column-based purification to provide a simple, rapid workflow for the isolation of inhibitor-free DNA from a variety of plant sources (Figure 1). So these three things (cell wall composition, secondary metabolites and turgor pressure) need advanced treatments. Use proteinase K overnight at room temperature or for 2 hours at 60C. << /Length 5 0 R /Filter /FlateDecode >> Modified CTAB Technique for Isolation of DNA from some Medicinal Plants By centrifugation, such co-precipitates can be removed in consecutive steps.The structure of CTAB. cold isopropanol. a vGX. ), and the procedures can be done in microfuge rather than big centrifuge (faster!). hb```Lf| The first is a small (3.5 kb) cryptic plasmid found in a high copy number in the marine species P. Leaves were disrupted using Qiagen TissueLyser II. 0 and nucleus wall. Genomic DNA extraction and amplicon generation. H'8R(|Zp{7OeCl _Y&!S5sUFF$>`! q;>:V^6FUW>!Aq2WF Notes from Kazakh Khanate till Political Dynamics + reading answers, Solutions - 6th Edition Computer and Networking, Stock Watson 3U Exercise Solutions Chapter 5 Instructors, A sample of letter of enumerator addressed to your employer, The Love Hypothesis Chapter 16 Adams POV by Ali Hazelwood (z-lib, Business Statistics and Mathematics Solved Past Papers, B.com Part 1 Punjab University 2009-2018, Siki soku 104 - dg sdg sdg fdg dsf sdg sdg sdg fdg dsf sdg sdg sdg fdg dsf sdg sdg sdg fdg dsf. PowerPoint Essential Training (Office 365/Microsoft 365) Prepare a working solution from stock. This method will help the researchers to efficiently isolate DNA from . By binding Mg with Follow this protocol on how to use PCI: Phenol, chloroform and isoamyl alcohol DNA extraction. CTAB Protocol for the Isolation of DNA from Plant Tissues. Previous studies demonstrated that Tannin is a proven PCR inhibitor that suppresses PCR amplification and remains bound with the DNA. Now let us come to our major player- CTAB. I already have explained the different types of polysaccharides and polyphenols present in plants and required chemical modifications accordingly. How to Extract DNA From Plant Leaves & Seeds | ZYMO RESEARCH I will also explain variations and concentrations of different CTAB buffers that you can try and use. RNA aptamers, specific for different proteins, have been exploited as biorecognition elements to develop specific biosensors (aptasensors). They are in general neutrophilic, strictly anaerobic hyperthermophilic peptide and sugar fermenters, that can reduce elemental sulfur to hydrogen sulfide (Palaeococcus may use ferric iron as electron acceptor), and they are rather easy to handle and to grow in the laboratory, probably because they are oxygen-resistant, if exposure occurs at room temperature or below. Nederlnsk - Frysk (Visser W.), Applied Statistics and Probability for Engineers (Douglas C. Montgomery; George C. Runger), How to extract plant DNA using CTAB method, 7 Chap 11 (Bioenergetic) F.Sc 1st Year Biology Helping Notes, L.N.Gumilyov Eurasian National University, Kwame Nkrumah University of Science and Technology, Jomo Kenyatta University of Agriculture and Technology, Constitutional law of Ghana and its history (FLAW306), Probability and statistics for engineers (MKT3802), Business Communication & Interpersonal Skills (MCM 509), Avar Kamps,Makine Mhendislii (46000), Power distribution and utilization (EE-312), Chapter 03 - The Time Value of Money (Part 1), SMA 2231 Probability and Statistics III course outline. iqy8D!fWJ64. This will increase the yield. PDF Principle of DNA Extraction - kau In addition, the presence of turgor pressure, all these things creates problems in plant DNA extraction. By whitelisting SlideShare on your ad-blocker, you are supporting our community of content creators. %%EOF Grow culture in 5 ml broth, pellet cells (~3000 g, 10 min), discard supernatant. choosing a selection results in a full page refresh. Muhammad I, Zhang T, Wang Y, et al. The Chemistry Behind Plant DNA Isolation Protocols | IntechOpen Evaluation of nematode DNA extraction methods for species Add liquid nitrogen to the tissue and roughly grind the sample into the mortar and pestle. Slowly pipette out and retain supernatant avoid interface material and lower phase. different method, providing an overview before delving deeper into the procedure in a step-by-step approach. In this article, we will discuss plant DNA extraction and CTAB buffer. membranes (such as those around the mitochondria and chloroplasts) have been broken recovery of dna from agarose gel, rajendra prasad central agricultural university. Nonetheless, to get success every time in plant DNA extraction, the first thing is to understand the structure and constituent of the plant cell of the species you are using. Plant samples (leaf, callus etc.) H|W~qbqh%oa7_$? H Now add chilled isopropanol (70%), and a pinch of NaCl to the supernatant and precipitate the DNA by inverting the tube several times. *n1cFPw (CTAB) contained in the extraction buffer. To make powder with liquid nitrogen or crush it in sterilized pestle mortar with 2 mL of This research evaluated five genome extraction methods including Protocol A (main CTAB), Protocol B (CTAB without -mercaptoethanol), Protocol C (CTAB without ammonium acetate), Protocol D (modified method of Murray and Thompson), and Protocol E (Gene All-Kit), to determine their ability to extract DNA from fresh and herbarium leaves of A. fridae. 0000000536 00000 n Activate your 30 day free trialto continue reading. In this study, we compared three different protocols of DNA extraction: NaCl (saline); phenol-chloroform and commercial kit (Promega)from three biological tissues of five individuals of Lutjanus purpureus under two methods of storage. It will surely make things easy. addition of glycine and/or ampicillin to weaken cells. It is imperative that sufficient cells are collected. In this sense, several studies have reported the efficiency of using cocoa leaf material for DNA extraction, however, has not been considered leaf developmental stage as an important variable for obtaining high quantity and quality of DNA. Purvi Jain - Perfumer - Sawai Fragrances | LinkedIn Wash with around 700 L of 70% ethanol. EB (Elution buffer): 5 mM Tris-HCl. DNA extraction is a process of isolating DNA. In contrast to the original method, the . Aboul-Maaty NAF, Oraby HAS. Free access to premium services like Tuneln, Mubi and more. We use cookies to track visits to our website, and we don't store any of your personal details. DNA precipitates, resembling a thread of translucent white snot, at the interface between the juice and alcohol. Do not sell or share my personal information, 1. 0000001976 00000 n Mix gently to precipitate the nucleic acid. CTAB is used in this method of DNA extraction. DNA, RNA, and Protein Extraction: The Past and The Present - Hindawi Store final DNA preparation at -20C. )Z$m|h_8"dOtv#P%w;:K&z]x.A`5.AZ%q9;eU! www.HelpWriting.net This service will write as best as they can. apart, the purification of DNA is performed. 22. PVP- PolyVinylPyrrolidone binds with the phenolic ring of tanning and prevents its interaction with DNA. Use the manufacturers guide for use. Kamber T, Malpica-Lpez N, Messmer MM, et al. Add 500 l 70% ethanol, resuspend pellet by flicking, allow to sit for ~5 min at room temp, then spin and drain again. Corpus ID: 102206113; Comparison of Four Methods of DNA Extraction from Needles of Pinus tabulaeformis @article{Ming2012ComparisonOF, title={Comparison of Four Methods of DNA Extraction from Needles of Pinus tabulaeformis}, author={Li Ming}, journal={Hubei Agricultural Sciences}, year={2012} } It appears that you have an ad-blocker running. The CTAB procedure would likely work with many gram-negative strains without modification, but gram positives would likely require the addition of lysozyme and proteinase K steps, and may also require modification of medium eg. Nguyen Quoc Trung. This protocol can be used on most gram-negative bacteria to obtain high-quality genomic DNA, which is both pure and very high molecular weight. (100 mM Tris-HCl pH 8, 2% (w/v) CTAB, 20 mM EDTA, 1 M NaCl, and 1% (v/v) Rapid isolation of high molecular weight plant DNA. 0000002394 00000 n Since DNA is insoluble in these alcohols, it will aggregate together, giving a pellet upon centrifugation. Add 500 l chloroform, vortex, spin 5 min, retain supernatant. DNA CHECK RUN: 11. After grinding, the tissue is transferred to a tube and CTAB buffer is added. 37 0 obj <> endobj Main steps in DNA isolation procedure: 4 Lysis Removal of proteins and contaminants Recovery of DNA A number of commercial DNA purification kits use the same principles as the manual method. This protocol is wholly adapted from the Coleman Lab protocols, including notes and comments. Centrifuge the precipitate for 10,000rpm for 2 minutes and collect the pellet (remove the supernatant). ARGOS8 variants generated by CRISPR-Cas9 improve maize grain yield under eld Codon p referance and factor influences it, How to approach supervisors for research opportunities, Direct Lineage Reprogramming: Novel Factors involved in Lineage Reprogramming, A common molecular basis for three inherited kidney, Presentation insect resistant transgenic crops ahmad madni (21-12-2016), Field application of metal microbe interaction, PPT for COT Methods of Cooking Poultry.pptx, CUNY Brooklyn College Diagnostic Museum Report Between Two Paintings.pdf, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. Carbohydrate, isolation and purification techniques. In addition, working with phenol and chloroform is also a biosafety hazard: Phenol can cause chemical burns and chloroform is a potential carcinogen.6,7 For many food testing labs, the use of these toxic chemicals is a major concern. INSTRUCTION: Proteinase K DNA extraction: 6. DNA was extracted directly from freshly harvested leaves ground in pre-heated CTAB buffer. So the first thing one should have to keep in mind is that depending upon the constituent of a plant cell wall, and the type of polyphenols they contain, prepare a buffer system. Centrifuge the sample at 25,000rpm for 5 to 8 minutes and transfer the supernatant into another tube. High molecular weight DNA yield in the range of 328 to 4776 ng/L with an average . Quality analysis of genomic DNA and authentication of fisheries After the tissue becomes a powder, add 500 L of CTAB extraction buffer and beta-mercaptoethanol, grind it again followed by vortexing for 5 minutes. Following the high demand of this product, several approaches have been suggested and developed aiming at the assessment of honey's quality and . Tris-HCl gives the solution a pH 4. After it becomes homogeneous, take the mixture into the 2ml Eppendorf tube. Lysis: Tissue grinder and use detergent. of Red Blood Cells, RBC. DNA extraction is relatively easy from citrus woody than non-citrus plants. Autoclave all the utilities prior to use including solutions. Select a plant species and prepare the protocol and chemicals accordingly. CTAB Protocol for Isolating DNA From Plant Tissues, the cetyltrimethylammonium bromide (CTAB) method, various modifications for different plant species, development and optimization of a one size fits all CTAB protocol difficult. However, TE can interfere with subsequent enzyme reactions (EB wont). Extraction(CTAB) Buffer 1.4 M Na Cl The Fast ID Genomic DNA Extraction Kit is designed for whole grains, fruits, and vegetables, and the Fast ID Ultra DNA Extraction Kit (Catalog No. The DNA extraction protocol can be completed in as little as 15 minutes and will give you a straightforward path to high-quality DNA. Plants bear secondary metabolites and a diversely rigid cell wall made up of polysaccharides like pectin, cellulose, chitin, etc. Screening of enrichment cultures of Thermococcales has revealed an unexpected diversity among VLPs (virus-like particles) in deep-sea hyperthermophilic environments. 0000002963 00000 n Discard all waste phenol and chloroform into chemical waste bottle in fume hood. The Cell A Molecular Approach Sixth Edition The Beauty of Science is to Make Things Simple, How to Choose a DNA and RNA Purification Kit, Picka DNA or RNA Purification Kit That Meets Your Needs, DNA Methylation Profiling and evolutionary history of modern man, How to get quality DNA for ChIP Sequencing. 1.05K subscribers The CTAB method yields high quality plant genomic DNA that can be used for virtually any application including genotyping and Illumina sequencing. CTAB - DNA precipitation DNA is hydrophilic and dissolves in water but not in alcohol To separate the DNA from the cell debris, alcohols and salts are used Organic solvents are used to separate the DNA to an aqueous phase, leaving lipids and proteins in the organic phase. Angeles JGC, Laurena AC, Tecson-Mendoza EM. The use of Urea is the easy and safest way to extract DNA from plants and sufficient enough for routine PCR and restriction digestion but not enough for sequencing or microarray analysis. The SlideShare family just got bigger. [,' u8p;le n0%j7giL (0xS6mnf6tW 2.8. 1. The final precipitate would be eluted and dissolved in the TE buffer. Treat the sample with PCI, centrifuge it, and collect the supernatant part. DNA extraction PANDORA For this DNA extraction. v;K6n8>0xx~fzNFLscB=)lo-s7iW:QxzA+ o45bW`=bN htt& Report on Carcinogens, Fourteenth Edition. You choose the most appropriate method ! 0000001112 00000 n %PDF-1.3 5. Total genome DNA from samples was extracted using the CTAB/SDS method . Conclusively, PVP and beta-mercaptoethanol must be there in the plant DNA extraction buffer with the CTAB. Other chemicals required in the process are SDS, protease enzyme, Phenol, chloroform, isoamyl alcohol and ethanol. It was adapted from Winnepenninckx B. et al., 1993, TIG: 9 (12), 407 (Technical Tips). Evaluation of five genomic DNA extraction methods for downstream Former website http://biogeek. Plant cells can be lysed with the ionic detergent CTAB, which forms an insoluble complex with In addition, the efficiency of PVP increases greatly by adding beta-mercaptoethanol. Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition, Techniques in Molecular Systematics and Evolution, Methods in molecular biology (Clifton, N.J.), Microsatellites for ecologists: non-radioactive isolation and amplification protocols for microsatellite markers, Molecular genetic analysis of recruitment and dispersal in the intertidal procelain crab, Petrolisthes cintipes, Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics, Biosensors for RNA AptamersProtein Interaction, Construction of plant bacterial artificial chromosome (BAC) libraries: an illustrated guide, 11 Deep-sea Thermococcales and their Genetic Elements: Plasmids and Viruses, Methods in Molecular Biology TM Methods in Molecular Biology TM PCR Protocols SECOND EDITION Edited by PCR Protocols SECOND EDITION Edited by. Extraction of DNA from Infected Cassava and Banana from Cameroon . Looks like youve clipped this slide to already. Academia.edu no longer supports Internet Explorer. Lysis of cell membrane: The first step of the DNA extraction is the rupture of the cell Only four small populations remain in Xue, Nanhu, and Cilai Mountains of Taiwan. Instead of fuzzy bands, clear and sharp bands can be achieved by using a pinch of CTAB into the SDS PAGE. 0000000812 00000 n if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[336,280],'geneticeducation_co_in-leader-2','ezslot_9',168,'0','0'])};__ez_fad_position('div-gpt-ad-geneticeducation_co_in-leader-2-0'); No universal CTAB DNA extraction protocol exists, keep in mind this thing. Prepare a 10X stock for CTAB solution to avoid repeated chemical preparation. KIT METHOD OF DNA EXTRACTION. dissolved in the aqueous solution are separated from the lysis homogenate. Once the tissue has been frozen, its ground into a fine powder with a mortar and pestle or a blender. "Processing with GMO detection method and confirmation of results for corn, soy, cotton and tomato."-Microorganisms' cultivation.-DNA extraction (CTAB ptotocol) -Method confirmation according to ISO 17025.-PCR (Polymerase chain reaction) . Cetrimonium bromide - Wikipedia PDF Heat Treatment of Bacteria: A Simple Method of DNA Extraction for ^ 0=pLRto6Q&N(Na*Y;"I$1k*wkX 5|AQ>4DWii/:&"E N %%Jb4jgAgluFd&Hw4[hply_.FnZL1ywbIWuB}^zl|pq. DNA quality was checked on 1% agarose gels using a ladder and the purity was checked as above. For instance, the DNA extraction setup and buffer which works for animal tissue, cant work for plants. I hope to test this at some point to confirm some of these comments, but as my source for many of these protocols, I know he is rarely, if ever wrong. EDTA, the activity of present DNase is decreased. I .g$!=n]BQ|nTr|0T5t4@XIR$fwyVIszC9Er(ze*RzoI$M%8FM=Mi fR{X[:[F R=XFBdvk>*H7&NIP'B.4GsXUS6j'^p2+#hY[xBYUJuk (L)4/vB`])tS2\YI!MSCKVN",NAN}/DKHFR 70C, 1 hour). . It is a major constituent of polyphenolic compounds and is almost present in all plants. Nonpolar molecules migrate into the organic phase and leave behind DNA and other polar molecules in the aqueous phase. The method needs to be modified for use on Gram-positives or yeast etc, by adding on extra lysis treatments at the front end of the protocol. Centrifuge the sample at 10,000rpm for 2 minutes and take supernatant to another tube. We have used this procedure successfully with Pseudomonas stutzeri strains which did not give good DNA preps with SDS-based DNA extraction methods due to polysaccharide production. In this study, we demonstrate a modified CTAB method for the isolation of DNA to maximize the quality and yield from small aphids. Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Do you know the CTAB can also be used in the SDS-PAGE? conditions, the detergent i. CTAB, which is more stable in alcohol than in water, can be washed Conclusion: Heat may be used to extract DNA from K . 4. Salting-out method. PDF DNA Extraction From Blood - KSU Learn faster and smarter from top experts, Download to take your learnings offline and on the go. t>V nk+ex!J};0R6B7wRR: %]?i=UXW0l4uRvREu?*js\2cWa>#T|n}%VV?WyuT"8}r6a}s8mP*bL {iWMs(U /M_]{VGZ"\+ If someone says it, its wrong. Add 500 l chloroform, vortex and mix thoroughly, incubate on ice 30 min. There are no repetitive and lengthy phase separation steps or hazardous reagents used, so you can further streamline your labs operations and protect the safety of key personnel. EDTA is a chelating component that among K6-0001-050 or -200) is designed for processed and/or multi-ingredient foods. Fast ID contains powerful denaturants and DNA-selective oBrgc;ntf24t``D7Sw8C&NQn"1_* ?dNOHhc/s3 ^D{6n>`SFcMedqW%MT0P!X?K` FlThc0 'wU/n;@wP~0ypKO8gW>LKn:?~hlfTvU=a"or^FuP]I.u;H\"X3Mh]buUbWQ5,N,0P+A+SXEAZV:57{F:999(G!Eh#`Ln{abDH"{JKiBH;jA} }9\cY"1La 2023 Zymo Research Corporation. See how we can help you extract high-quality plant DNA, in less time. The more finely your tissue is ground, the more efficient you DNA extraction will be, making this a critical step for successful DNA extraction. CTAB solution: 3% CTAB, 1.5 M NaCl, 10 mM Na 3 EDTA, 0.1 M MOPS-acid; DNA must be purified from cellular material in a manner that prevents degradation. PRESENTED BY: AYESHA KABEER DNA was diluted to 1 ng l 1 with sterile distilled water. CTAB protocol for the isolation of DNA from difficult tissues (high levels of secondary metabolites or polysaccharides), herbarium and soil. The successive treatment with 70% ethanol allows an Among the Thermococcales, the most important order of the phylum Euryarchaeota, only one virus is isolated and completely characterized to date. x$)FcD2k@ c@5#3Q`e^KDzqjpcm|;lm+ou>oyu?mei{:.||vtz~mG18zh]w \v?o.?;]wWv:auaw:t@-Og8ArNg(quz|JFv\'nC~w^P2uwpvRwghEPC"w;x@wb:^Q$~P9&*RlJAWfb:wT]8PJ\*u;Tut7.TJOD$F:ZTi/=l6QMoT 7j!Fq6bIXuO07:Ro2St~Ovt4',b6bJQ\v:"E 37 12 The DNA pellet do not dry and dissolved immediately in 300 l 1xTE, pH 8.0 at 55C for 5-10 minutes. Plate-grown cells are fine also growth scraped from half of a streak-plate is about the same amount of biomass as a fully-grown 5 ml broth. The DNA was used in nested or single PCR assays to detect the 16S rRNA and SAP genes using the phytoplasma 16S rRNA universal primer pairs P1/P7 and R16F2n/R16R2 (Deng & Hiruki . The following stock solutions and reagents were prepared by mixing the standard amount of chemicals. DNA Extraction and Purification - labome.com polysaccharides in a high-salt environment. Elution buffer (EB) is just Tris-HCl, which gives the DNA some protection due to its pH buffering. There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) disruption of the cellular structure to create a lysate, 2) separation of the soluble DNA from cell debris and other insoluble material, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other Cornejo OE, Yee MC, Dominguez V, et al. But differences in the cell structure and chemical composition of a cell require a different DNA extraction setup. Plan your day carefully and set aside the proper amount of time to complete the entire protocol. CTAB Protocol for the Isolation of DNA from Plant Tissues However, other chemicals like RNase, proteinase K, SDS and PCI may be used to improve the quality and yield of DNA. Why is DNA extraction from plants difficult? There are two reasons for that. Magnetic bead-based DNA extraction: 8. 2. Effect of geographic isolation on genetic variation and population ^ cQmG;Q5h7~S.KC10NdXwkx|%!UPvDPXN2_82q?Lz>O&=pq7^9vmA(PdLe m C1!j 3SK97vU|(TgEw'q>jh:k"a6==UDnMW+6i4~t5F&CfYn*OGB"GrL#~]mT$zUIa 6/iDbe h'*omlb_i*G'*MBP q08C q~x]ufAi=Y a3FanN(CSQY6YvQ*K=($b6(+R@6}IDBDD+MD]R@+?%|C,Hi C}dFf13WI ve^*G. Dissolve in 50 L 10 Secret Tips for DNA Extraction to Get Good Results. endstream endobj 38 0 obj <> endobj 39 0 obj <>/Font<>/ProcSet[/PDF/Text]>>/Rotate 0/Type/Page>> endobj 40 0 obj <> endobj 41 0 obj <> endobj 42 0 obj <> endobj 43 0 obj <> endobj 44 0 obj <> endobj 45 0 obj <> endobj 46 0 obj <> endobj 47 0 obj <>stream You can read the details below. Edwards et al. Get news, product info, tips, industry updates, events, freebies, and more delivered right to your inbox. We've encountered a problem, please try again. if(typeof ez_ad_units!='undefined'){ez_ad_units.push([[250,250],'geneticeducation_co_in-leader-1','ezslot_23',145,'0','0'])};__ez_fad_position('div-gpt-ad-geneticeducation_co_in-leader-1-0'); Homogenization is a process to prepare a homogeneous mixture of plant tissue using tissue homogenization techniques like physical grinding using a Mortar and pestle. Thus even if your DNA is good and your PCR fails repeatedly, you would have to worry about tannin. We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. METHODS OF DNA EXTRACTION - SlideShare Instant access to millions of ebooks, audiobooks, magazines, podcasts and more.