/Type /ExtGState banana dna extraction lab report conclusion - chrissihart.com f MATERIALS Fresh Bananas Droppers Water 70% rubbing alcohol 3 Plastic Bag (Ziplock) Salt Liquid Dish Soap Mixing bowl (Medium size) Measuring spoon Measuring cup 3 Beaker (Big and ASU - Ask A Biologist. HSj@WcczG]A)-84H$$`;:4yofRSLGiqU"M l 8cjzR. PDF Banana dna extraction lab report conclusion You share 50 percent of your DNA with each of your parents. Body identification is a subfield of forensic science wherein investigators need to identify a body. DNA extraction The concentrations of the extracted DNA was measured using the NanoPhotometer,UV/Vis spectrophotometer (Implen, munich, Germany) and the average concentration for 12 samples was 0.045g/l. DNA can be extracted and observed. 5 0 obj * To investigate the effect of temperature on DNA extraction from bananas. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The book is also an excellent resource for those studying or researching food technology, food science, food engineering, food packaging, applied nutrition, biotechnology, and more. The positively charged sodium ions in the salt help protect the negatively charged phosphate groups that run along the backbone of the DNA. 0000002685 00000 n We succesfully investigated the effect of temperature on DNA extraction from bananas. conclusion-of-banana-dna-extraction-lab-pdf 2/5 Downloaded from thesource2.metro.net on February 15, 2023 by guest products. Banana and Strawberry image by Ralph Daily via Wikimedia Commons. %&'()*456789:CDEFGHIJSTUVWXYZcdefghijstuvwxyz 0000003225 00000 n The nuclei acid DNA or deoxyribonucleic acid serves as the genetic material in all living organisms as well as in most viruses. After the alcohol layer is set up, wait for eight minutes. What is the formula for calculating solute potential? %PDF-1.5 Kiwis and bananas both together produced the same amount of strawberries alone. The process involves a few general steps, including mashing, filtration, precipitation, and extraction. Overall, the Lab: Extracting DNA from Bananas demonstrated and visualized the process of how DNA can be seen from the naked eye by removing DNA directly from bananas. 5) Ef+\:IZy}r(| F @q!4}g' U-CBb8sxurgg$t@AumUg Sx}Pa=cWyyh>WikxqB(;I&dCf&7CgCr(zMQl`PL BXIfBk. Save time and let our verified experts help you. cookie policy. In the cytoplasm, the information in RNA is translated into proteins, which serve as the end products of most all genes. If the steps were altered then the yield or the amount of DNA per banana could have been very small because the DNA would get trapped inside the cells or bound to lipids. Strawberries are octoploid, which means they have eight copies of the DNA in their genome in every cell! Why or why not? DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. %%EOF The filtrate, or filtered solution from the bag, was then placed into a test tube. Altering Animals. 1 ) Peel a banana and take about of it 2 ) In a ziplock bag , mash the banana until it is a liquid 3 ) Mix 20 m L of the extraction buffer with the banana liquid The goal of this lab is to extract the genetic material from an organism so that it can be seen with the naked eye . Write reflection paper about this question. This DNA extraction is simple and works every time but the DNA is not pure. You are looking at DNA! Segments, or pieces, of DNA are called "genes." Hear Let this two-layer mixture sit for eight minutes. The bananas were mashed for about 5 minutes. Genes, along with other parts of our DNA that turn genes on and off, hold information for how our body develops and functions. (PDF) SCIENCE LAB REPORT How does the amount of detergent affect the Performance cookies are used to understand and analyze the key performance indexes of the website which helps in delivering a better user experience for the visitors. You must cite our web site as your source. Water is a molecule of two hydrogen atoms and one oxygen atom (H2O)more. Watch biologist Melissa Wilson Sayres as she shows you step-by-step how to extract DNA from a banana. The mixture was divided into two 50ml conical flasks (20ml each). * To properly and successfully extract DNA from banana using cell disruption and separation techniques. Extraction solution S was added into ziplock Extraction 1 and extraxtion solution SS into ziplock bad Extraction 2. Motivation: Recent technological promotions in the field of genomics have resulted in Following Generation DNA Sequencing Technologies. Other uncategorized cookies are those that are being analyzed and have not been classified into a category as yet. When you are done, take a closer look at the stuff on the stirrer. Extracting DNA from a Banana and Other Fruits Introduction All living things, bananas and people included, pass on information from one generation to the next using the same basic material, DNA. These comprise the genetic alphabet which in various combinations, will specify the components of proteins. We will use common household products to break apart the cells in a banana and extract out the DNA. Conclusion Of Banana Dna Extraction Lab Pdf [PDF] - thesource2.metro 0000045111 00000 n /SM 0.02 In order to extract DNA you must macerate a thumb sized piece of Banana in a zip lock bag.Then weigh out 1.0 gram of salt and 2.0 grams of meat tenderizer the continue maceratingMeasure out 9.0 mL of water and add to the bag.Then continue macerating.See Ms.Leavitt to get 1mL of detergent soap then continue macerating.Once a thick pace called endobj % Banana DNA Extraction by aaliyah jones - Prezi The salt contributes positively charged atoms that neutralise the normal negative charge of DNA. collected. Approximately 2ml of cold 95% ethanol was added into each test tube. Its natural shape is called a double helix and when seen under extremely high-powered microscopes, it looks kind of like a ladder twisted into a spiral shape. 2 What are the benefits of extracting DNA? Breaking cells open to release the DNA. The test tube solution. DNA I have also made the conclusion that filter paper is a neccissity. DNA extraction is also the first step in genetic engineering of animals. PDF Extracting DNA from Bananas and Strawberries Purpose - New Paltz Middle All the methods employ agarose gel electrophoresis to separate the DNA molecules. 4.1. As part of the chromosomes, the information contained in genes can be transmitted faithfully by parents through gametes to their offspring. You may see some bubbles and cloudy material moving around in the alcohol. Equipment You will need the following: Two clear glasses/cups Sealable plastic bag Banana Blunt knife and teaspoon Plate/chopping board Measuring jug 0000040917 00000 n /ca 1.0 0000002160 00000 n In addition, there are four kinds of nitrogenous bases in DNA. support@phdessay.com. DNA needs a lot of DNA for the extraction. These cookies track visitors across websites and collect information to provide customized ads. the DN +co$or' texture', The soap so$ut#o" co"ta#"s so%#um $aure$ su$!ate' wh#ch, the soap pu$$s apart the !ats +$#p#%s a"% prote#"s that. ASU - Ask A Biologist. Remove and discard the used coffee filter. This could be a problem if two bacteria looked similar because they could easily. Descr#be the appeara"ce o! During the DNA extraction, the soap pulls apart the fats (lipids) and proteins that make up the membranes surrounding the cell and nucleus. 20g of banana was added into each ziplock bag labeled 'Extraction 1' and 'Extraction 2' 3. Why do you put liquid soap in a banana strainer? The cookies is used to store the user consent for the cookies in the category "Necessary". Two of the nitrogenous bases, adenine and guanine, belong to a group of compounds known as purines. 8. <>>> xnFab5\8CA _4E$`i,yI:=x-Yd.5g:#6}^={s/0lH*|?x'}=]4}vb ~M4q'(OV%l`! Although nowadays very few errors are made due to scientists using biotechnology equipment that allows them to look at DNA to see exactly how organisms are different, and find out how they work. 7 0 obj If I mash up a living organism , then I can isolate the DNA . The order in which the procedure is stated is very important because the cells must first be broken down in order to release the DNA to be seen. 1 2 . The soap breaks apart the cell membrane and the cell parts that hold the DNA. For instance, the detergent or soap helps to break down the cells outer membrane, and the salt helps to separate the DNA from other materials in the cell. Presentation for the Living Faith Series, May 16, 2021 The solution had to be blended for 15 to 20 seconds, until it became a dense mixture. The banana DNA was circular and was very separated into many smaller pieces. How does detergent help to break down DNA? Question 16 options: All of the above Improve crop production Improve disease resistance Generate new, 1 ________ is seen when ABCDEFGHI becomes ABCFEDGHI. In 1944, a group of scientists led by Canadian biologist Oswald Avery performed an experiment that would determine which molecule in a heat-killed bacterium was most important for transformation to occur. 0000011509 00000 n (B) Banana Extraction 1. $#*u#, bamboo s)ewer +a tw#r$#"g mot#o" wor)s best. Place the coffee filter in a clear glass cup, securing the top of the filter around the lip of the cup. Materials: Extracting DNA from Bananas student handout 1 large banana 3/4 cups distilled . You also have the option to opt-out of these cookies. The two strands are held together by hydrogen bonds between adenine and thymine and between guanine and cytosine. Dna-Rna Extraction, Lab Report Example | essays.io 5. Do this for 30 to 45 seconds. &t #s, #"cre%#b$y sma$$' but we ca" see #t by extract#"g DN a"%, %#st#$$e% water #" the p$ast#c bag' sea$ the bag a"%, so$ut#o" s$ow$y !or about a m#"ute u"t#$ the shampoo, the empty cup. 135 32 Seeing Banana Banana DNA Extraction Activity Ask A Biologist activity for classroom and home Table of Contents Activity Overview Materials Review of Steps and What is Happening Instruction sheet For Teachers Next Generation Science Standards, Arizona Standards, Do-It-Yourself DNA | Science Project - Science Buddies DNA is organized into genes and stores genetic information. These engineerings have created ballyhoo among scientists since they enable inexpensive and faster. 9 How does detergent help to break down DNA? For the genes DNA to subsequently influence an inherited trait, the stored genetic information in the DNA in most cases is first transferred to a closely related nuclei acid, RNA or ribonucleic acid. It holds the instructions necessary for the organism to grow and function, and is passed on from generation to generation through heredity. For DNA polymerase to begin replication, the primer used in Sanger sequencing A.can have any nucleotide sequence B.will have a complementary sequence to the 3 end C.will have a. Mashing the banana exposes a greater surface area from which to extract the DNA. Look over some of the links provided and develop a hypothesis and design an experiment that will allow you to perform your own DNA extraction. PDF Ask A Biologist - Banana DNA Extraction - Activity Figure 1. Variants of genes, called alleles, are responsible for differences in hair color, eye color, and earlobe shape. REFERENCES: BOOKS: * Neil A. Campbell, Jane B. Reece, Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Robert B. Jackson, Biology (8th Ed), Pearson International Edition: Pearson, Benjamin Cummings. And because DNA doesn't dissolve in alcohol, this substance helps DNA to cluster into a separate layer. DNA stands for deoxyribonucleic acid, which is a nucleic acid that contains the sugar deoxyribose. These cookies help provide information on metrics the number of visitors, bounce rate, traffic source, etc. Furthermore, analysis of forensic evidence is defined as the application of forensic science and technology to identify specific objects from the trace evidence they leave, often at a crime scene or the scene of an accident. Cells reproduce in part by passing deoxyribonucleic acid (DNA) from parent cells to offspring cells. xref What is the best fruit to extract DNA from? At an absorption of 280 wavelengths, the DNA measured 3.802 using a 374.66 ng/ul sample. assignments. Result Photo 1: Test tubes containing solution S and SS CONCLUSION. This essay was written by a fellow student. However, it is nice because the students get to see and hold in their hand the substance they have been studying that seems so abstract and hard to believe it even exists. Banana Dna Extraction Lab Worksheet Answers - Blogger . DNA is insoluble in low molar salt solutions but soluble in low molar salt solutions thus keeping the DNA in solution. The probable reason for the tinted brown shade in the human epithelial DNA and not the strawberry DNA is that human epithelial DNA is extracted from one's mouth in which. 0000044903 00000 n 48 Vitosha Boulevard, ground floor, 1000, Sofia, Bulgaria Bulgarian reg. And because the DNA doesnt dissolve in alcohol, this substance helps the DNA clump together in a separate layer. 1. 2 Mar 2023. https://askabiologist.asu.edu/activities/banana-dna. /Width 625 >> Extracting Dna From A Banana Experiment | PDF - Scribd Lab Report Banana Dna. The liquid soap is added to help break down cell membranes to release the DNA. The salt was dissolved by stirring slowly to avoid foaming. /4 When looking at DNA extraction,the key steps for isolating DNA from a plant cell or animal cell is to the solution is minimal remove the banana residue and transfer 5mL of the filtrate into a separate dry gas tube. 0000008293 00000 n Within every living organism, most cells contain a complete set of DNA instructions. Some additional protocols for DNA extractions: Sex Influenced Female Dominant Inheritance, beakers 100ml and 2x250ml (or jars that same size), liquid dishwashing detergent (clear solution works better than cloudy), Make a 10% solution of dishwashing detergent in one of your 250ml beakers (make enough to fill the mortar half way), Add the 10% detergent solution to cover the fruit, Grind the banana in the soap solution until there are no lumps (this is the hardest part, have the students take turns), Fasten a coffee filter to the 250 ml beaker with a rubber band so there is room for the fruit to be poured into the filter, Pour the fruit and soap mixture into the filter and place in a cold place to filter, Clean up your lab area while you are waiting for the filtering to complete, Remove the filter and throw away the contents, Pour enough of the liquid into a test tube to fill it 2 cm high, Add twice the volume of 95% ethanol (try to layer the alcohol on top by gently pouring it down the side of the test tube), The DNA will precipitate in the boundary between the alcohol and the banana mixture as a cloudy mass. Because DNA exists inside of cell and ultimately inside of the nucleus proteins must be denatured to locate the DNA. 0000039985 00000 n The DNA precipitates out of the solution when the alcohol is added. 9. xbbbd`b``3] Extracting DNA from Bananas In the Lab: Extracting DNA from Bananas, DNA was removed from bananas that had been blended with water in order to examine how DNA is seen from the naked eye. Complementarity serves as the basis for both the replication of DNA and for the transcription of DNA into RNA. Step 1. The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". DNA is a component of living and once-living things. Why is working with linked genes not simple? The salt enables the DNA strands to come together, or aggregate. 20g of banana was added into each ziplock bag labeled Extraction 1 and Extraction 2 3. This complementarity between adenine and cytosine nitrogenuos base pairs and between guanine and cytosine nitrogenuos base pase pairs, attracted to one another by hydrogen bonds, is critical to genetic function. Approximately 3ml of banana solution were dispenced into each test tube. With Retrieved March 2, 2023 from https://askabiologist.asu.edu/activities/banana-dna, Melissa Wilson Sayres. Traditional methods include judging by phenotypes characteristics. Potential of independent assortment in meiosis I C. They reside on different chromosomes D. One gene often. Did you know that we have over 70,000 essays on 3,000 topics in our Squishy Science: Extract DNA from Smashed Strawberries with free plagiarism report. 3g PROCEDURE (A) Extraction solution recipe: 4ml of shampoo was mixed with 36ml of distilled water. 4 0 obj ASU - Ask A Biologist.